The BRAF/CEN7 probe is located on chromosome 7. A 1300 Kb probe covering the BRAF gene region at 7q34 is labeled with an orange dye, and part of chromosome 7 (CEN7) is labeled with a green dye.

BRAF mutations are important therapeutic targets in multiple solid tumors, with varying mutation rates in melanoma, thyroid cancer, colorectal cancer, and NSCLC. It is also a molecular subtype of central nervous system gliomas, and this fusion is found in pilocytic astrocytoma (common), pilomyxoid astrocytoma (sellar region), and diffuse leptomeningeal glioneuronal tumor.