The DDIT3 probe consists of an orange-labeled probe hybridizing proximal to the DDIT3 gene region at 12q13.3 and a green-labeled probe hybridizing distal to the DDIT3 gene region at 12q13.3.

DDIT3 FISH probes detect the DDIT3 (CHOP) gene rearrangement at 12q13, primarily serving as the gold-standard diagnostic test for myxoid liposarcoma. They also aid in distinguishing this tumor from other sarcomas with round cell morphology and detecting the specific *FUS-DDIT3* or *EWSR1-DDIT3* fusion transcripts critical for confirming the diagnosis. This technique is essential for accurate sarcoma subtyping and guiding clinical management.