The PD-L1/ABL1 probe is located on chromosome 9. A 740kb probe covering the PD-L1 gene region at 9p24.1 is labeled with an orange dye, A 1050kb probe covering the ABL1 gene region at 9p34.13 is labeled with a green dye. Detects PD-L1 gene amplification in various cancers for immunotherapy selection and prognosis. Used to identify patients with PD-L1 overexpression who may respond to PD-1/PD-L1 checkpoint inhibitors. Essential for predicting therapeutic efficacy in lung cancer, hepatocellular carcinoma, and other malignancies. ABL1 component enables detection of chromosomal aberrations in hematologic disorders.